Purification and Properties of Pyridine Nucleotide-Independent l -Lactate Dehydrogenase from Polyporus circinatus
نویسندگان
چکیده
منابع مشابه
The D-galactose oxidase of Polyporus circinatus.
The mold Polyporus circinatus produces an extracellular oxidase that catalyzes the oxidation of n-galactose (1). The reaction appears superficially to be similar to the oxidation of n-glucose catalyzed by notatin (2)) and can be followed by measuring the consumption of oxygen or the production of Hz02 (1). The enzyme is useful for the determination of galactose, employing the same peroxidase-ch...
متن کاملPurification of reduced pyridine nucleotide dehydrogenase from human erythrocytes and methemoglobin reduction by the enzyme.
Reduced pyridine nucleotide dehydrogenase was purified 44,000-fold from normal human erythrocytes by procedures including ammonium sulfate fractionation, calcium phosphate gel chromatography, Sephadex G-100 gel filtration, and isoelectric focusing. The most purified enzyme preparation showed a single homogeneous peak (s~~,~ = 2.77 S) upon ultracentrifugation and was nearly homogeneous on acryla...
متن کاملNAD-Independent L-Lactate Dehydrogenase Is Required for L-Lactate Utilization in Pseudomonas stutzeri SDM
BACKGROUND Various Pseudomonas strains can use L-lactate as their sole carbon source for growth. However, the L-lactate-utilizing enzymes in Pseudomonas have never been identified and further studied. METHODOLOGY/PRINCIPAL FINDINGS An NAD-independent L-lactate dehydrogenase (L-iLDH) was purified from the membrane fraction of Pseudomonas stutzeri SDM. The enzyme catalyzes the oxidation of L-la...
متن کاملGalactose oxidase of Polyporus circinatus: a copper enzyme.
In 1959, Cooper et al. (1) described the isolation of an enzyme from Polyporus circinatus Fr. which catalyzed the oxidation of galactose by molecular oxygen. MTe have previously described a procedure for the purification of such an enzyme from culture filtrat,es and have shown the reaction product to be D-g&CtOhexodialdose, oxidation occurring at the C-6 rather than the C-l position (2). The pr...
متن کاملPhotosynthetic pyridine nucleotide reductase. I. Partial purification and properties of the enzyme from spinach.
In 1939, Hill (1) reported the fundamental discovery that isolated chloroplasts possess the ability to affect a portion of the over-all reaction of photosynthesis; namely, the photolysis of water. In the original experiments of Hill, ferric oxalate was used as the hydrogen acceptor. During the past 20 years, a variety of substances has been found to function as oxidant for the Hill reaction (2)...
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ژورنال
عنوان ژورنال: Journal of Bacteriology
سال: 1974
ISSN: 0021-9193,1098-5530
DOI: 10.1128/jb.119.3.1000-1005.1974